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Objective To establish an improved model of exercise-induced glycometabolism in type II diabetic rats,and to provide a theoretical reference for the establishment of exercise prescription for type II diabetes.Methods Forty-five 8-week old SPF male Wistar rats were used in this study.Of which 32 were fed with high-fat diet for 7 weeks,and intraperitoneal injection of 30 mg/kg STZ was given to establish the rat model of type II diabetes.The normal rats and successful model rats were divided into four groups:The normal control group (C group),normal exercise group (CE group),diabetic group (DM group) and diabetic exercise group (DME group).The exercise group was assigned by the Ploug training protocol,6 days/week,60 min/day,for a total of 8 weeks.After the high fat diet fed for 7 weeks,blood sample was taken from the tail vein,FBG and serum insulin were detected after baseline and 8 weeks exercise,and blood sample was collected from the tail vein to determine the FBG.Serum insulin (FINS) was detected by orbital blood sampling at the end of 8 weeks of exercise,and HOMA-IR was calculated.Results 1.After 7 weeks of high fat diet,compared with the groups C and CE,the levels of FBG,FINS and HOMA-IR were significantly higher in the DM and DME groups.2.After 8 weeks of exercise intervention,compared with the groups C and CE,FINS was significantly lower in the groups DM and DME,but the FBG and HOMA-IR were higher.Compared with the DM group,the level of FINS was significantly higher in the DME group,and the levels of FBG and HOMA-IR were significantly lower.The body weights of DM and DME groups were significantly lower than those of the groups Cand CE,the body weight had no significant difference between the DME and DM groups,and similar result was between the groups CE and C.Conclusions 1.The rat model of type II diabetes is successfully established with high fat diet for 7 weeks plus STZ injection(30 mg/mL).2.Aerobic exercise 60 min/day for a total of 8 weeks can improve the glycometabolism in type 2 diabetic rats,to be an ideal animal model for study of the mechanism of prevention and amelioration of type II diabetes.
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AIM:To explore the influence of long-term swimming on peripheral neuropathy in type 2 diabetic rats.METHODS:Male Wistar rats were fed with a high-fat and high-fructose diet, and injected with streptozocin to estab-lish a model of type 2 diabetes mellitus.The rats were randomly divided into 4 groups:blank control group (C group), ex-ercise control group ( CE group ) , diabetes mellitus group ( DM group ) and diabetes mellitus +exercise group ( DME group).The rats in CE group and DME group received 8-week swimming training (6 d/week).The training time was 20, 30 and 45 min in the first 3 d,respectively, and then it increased to 60 min a day.Eight weeks later, the motor nerve conduc-tion velocity ( MNCV ) and the levels of tumor necrosis factor α( TNF-α) , interleukin 6 ( IL-6 ) and C-reactive protein ( CRP) in sciatic nerve tissues of the rats were measured .The morphological changes of the sciatic nerve were also observed under light microscope .RESULTS:Compared with DM group , 8-week swimming obviously accelerated the MNCV ( P0.05).The obvi-ous nerve injury in DM group was observed .However , the pathological change of the sciatic nerve in DME group was re-lieved.CONCLUSION:Eight-week swimming training significantly accelerates the MNCV , attenuates the nerve injury in diabetic rats and has protective effect on peripheral nerve , which may be correlated with relieving the inflammatory reaction .
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BACKGROUND: Exercise preconditioning can lighten exercise-induced muscle damage, thereby to avoid delayed onset muscle soreness. At present, experimental research is scarce that apply intedeukin-6 (IL-6), creatine kinase (CK) and creatine kinase isoenzyme (CK-MM) to evaluate skeletal muscle damage.OBJECTIVE: To observe the effects of exercise precondiUoning on muscle damage at different phases after eccentric exercise and changes of blood IL-6, CK and CK-MM.DESIGN, TIME AND SETTING: The randomized control animal expedmant was carded out in the Animal Laboratory of Chengdu Sports University between 2006 and 2007. MATERIALS: Eighty female adult SD rats, weighing (231.3+12.44) g, were adopted. Eighty rats were randomly divided into without exercise preconditioning group (n=40) and exercise preconditioning group (n=40). Each group was assigned into 5 subsets, termed before exercise, immediately after exercise, 24, 48, 72 hours after exercise, with 8 rats in each subset. METHODS: Except before exercise subset, other rats in the without exercise preconditioning group were forced to do treadmill exercise (19-21 m/min, -16° incline, 90 minutes). All rats of exercise preconditioning group were forced to do eccentric treadmill exercise for two weeks. After two weeks, treadmill test was made for rats except before exercise subset. MAIN OUTCOME MEASURES: Soleus muscle structure, blood IL-6, CK and CK-MM immediately, 24, 48, 72 hours after eccentric exercise.RESULTS: The soleus muscle was damaged after exercise, especially in without exercise preconditioning group at 24-48 hours after exercise. Blood IL-6 of without exercise preconditioning group increased significantly immediately after exercise and then gradually decreased, but again raised at 72 hours after exercise. In the exercise preconditioning group, blood IL-6 slightly reduced immediately after exercise and then gradually increased. Peak value appeared at 48 hours. After exercise, IL-6 of exercise preconditioning group was obviously lower than that of without exercise preconditioning group. Before exercise, serum CK and CK-MM of exercise preconditioning group were less than that of without exercise preconditioning group. After exercise, the CK and CK-MM were firstly raised and then reduced in two groups. Except 72 hours after exercise subset, the variation of CK and CK-MM of exercise preconditioning group was lower than that of without of exercise preconditioning group. CONCLUSION: Exercise preconditioning is redounded to lighten the ultrastructure injury of skeletal muscle induced by eccentric exercise and blood indices changes induced by exercise stress. The individual variation of CK and CK-MM is so tremendous that they fit the comparison of intrasubject variability.
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BACKGROUND: Aerobic exercise is a popular and effective way of fitness. All kinds of changes occur in the human system, especially the changes in the cardiovascular system. Enhancing the cardiovascular system functions is the most important role in the aerobic exercise. OBJECTIVE: To sum up effects of aerobic exercise on blood indicators, cardio-vascular function. RETRIEVAL STRATEGY: A computer-based search was conducted in Pubmed for literatures related to aerobic exercise and cardiovascular system published between January 1998 and December 2006 with the key words of "aerobic exercise, cardiovascular" in English. Simultaneously, China Journal Full-text Database was looked for relevant articles published from January 2001 to December 2006 with the same key words in Chinese. The data were selected firstly. Inclusive criteria: ①the influence of aerobic exercise on cardiovascular function, ②influence of aerobic exercise on blood indexes. The exclusive criterion for deletion was the repetition of the same research. There were 81 literatures in accordance with the above-mentioned criteria. LITERATURE EVALUATION: 51 papers of repetitive research were excluded, 30 of them were accorded with the inclusive criteria and were included. DATA SYNTHESIS: Aerobic exercise plays the most important role in fitness. Enhancing the cardiovascular system functions is the most important role in the aerobic exercise. Epidemiological studies and experimental studies have indicated that aerobic exercise not only can increase energy consumption, improve vitality lipoprotein and lower blood lipid content, but also can reduce blood pressure in patients with hypertension and decrease the incidence of arteriosclerosis and other cardiovascular diseases, and can effectively enhance cardiac function. CONCLUSION: Appropriate aerobic exercise can lead to the enhancements of cardiac function and vascular function, and plays an important role in the prevention and treatment of cardiovascular diseases.
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This research is designed to detect the dynamic changes of microcirculatory ultrastructure and the MDA content, SOD and GSH-px activity in rat cerebrum immediately, 24h and 48h after exhaustive swimming. The results showed that there were obvious changes of ultrastructure in rat cerebral microcirculation immediately after exhaustive swimming. The changes were resumed in some degree after 24h and recovered almost to the normal after 48h. Immediately after exhaustive swimming, the MDA content did not change obviously; the activity of SOD increased markedly, and then it decreased markedly after 24h, but it increased again after 48h. The activity of GSH-px descended markedly after 24h; it descended synchronously with the decrease of SOD activity, showing that the consumption of SOD and GSH-px should be the most at this time. Therefore, the free radical should be removed in time for reducing the cerebral injury and promoting the recovery of cerebral microcirculation.
Subject(s)
Animals , Male , Rats , Brain , Metabolism , Capillaries , Cerebrovascular Circulation , Glutathione Peroxidase , Metabolism , Malondialdehyde , Metabolism , Physical Exertion , Rats, Sprague-Dawley , Superoxide Dismutase , MetabolismABSTRACT
This article summarizes the effects of intense exercise on the structure and function of the kidney,the possible mechanism of the changes of the structure and function of the kidney and the research state of the improvement of the function of the kidney by the medicine.The research suggests the intense exercise will lead to the damage of the renal tubules and the glomerulus,the increase of the LPO of the kidney tissue,the decrease of the activity of SOD,the appearance of the exercise-induced albuminuria,exercise-induced hematuria.Vitamine E,Vitamine C,GSH have some protective effect on the structure and function of the kidney.
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12.08g herbs/kg.The overall effective rate for coronary heart disease was86.8%.CONCLUSION:Danzhisuxin capsule is appropriate in formulation and feasible in preparation technique.The method of quality control is reli?able.No toxic reactions were found and the clinical effect was satisfactory.